Structural insights into conservedl-arabinose metabolic enzymes reveal the substrate binding site of a thermophilicl-arabinose isomerase
نویسندگان
چکیده
منابع مشابه
L-arabinose Negative Mutants of the L-ribulokinase Structural Gene Affecting the Levels of L-arabinose Isomerase in Escherichia Coli.
C @ 51 37 19 12 5 42 3 49 57 21 59 1.U 1 1 1 1 l 1 1 1 WENTY-two L-arabinose nonutilizing mutants of Eschrichia coli B/r have Tbeen ordered with regard to each other and a closely linked leucine marker by three-point tests using transduction of E . coli by phage Plbt (GROSS and ENGLESBERG 1959; ENGLESBERG, ANDERSON, WEINBERG, LEE, HOFFEE, HUTTENHAUER and BOYER 1962; BOYER, ENGLESBERG and WEINBE...
متن کاملAnalysis of the arabinose-5-phosphate isomerase of Bacteroides fragilis provides insight into regulation of single-domain arabinose phosphate isomerases.
Arabinose-5-phosphate isomerases (APIs) catalyze the interconversion of d-ribulose-5-phosphate and D-arabinose-5-phosphate, the first step in the biosynthesis of 3-deoxy-D-manno-octulosonic acid (Kdo), an essential component of the lipopolysaccharide in Gram-negative bacteria. Classical APIs, such as Escherichia coli KdsD, contain a sugar isomerase domain and a tandem cystathionine beta-synthas...
متن کاملHeterodimers reveal that two arabinose molecules are required for the normal arabinose response of AraC.
AraC protein, which regulates expression of the l-arabinose operon in Escherichia coli, is a dimer whose DNA binding affinity for pairs of DNA half-sites is controlled by arabinose. Here we have addressed the question of whether the arabinose response of AraC requires the binding of one or two molecules of arabinose. This was accomplished by measuring the DNA dissociation rates of wild-type Ara...
متن کاملStructural study of hinge bending in L-arabinose-binding protein.
The L-arabinose-binding protein of Escherichia coli is a periplasmic component of the bacterial L-arabinose transport system. The three-dimensional structure of the molecule has been determined by x-ray diffraction and shown to have two globular domains and a connecting hinge. Theoretical study of the flexibility of the hinge using computer simulation showed that the hinge is quite permissive i...
متن کاملEscherichia coli YrbH is a D-arabinose 5-phosphate isomerase.
A gene encoding for arabinose 5-phosphate isomerase (API), which catalyzes the interconversion of d-ribulose 5-phosphate (Ru5P) and d-arabinose 5-phosphate (A5P), has been identified from the genome of Escherichia coli K-12. API is the first enzyme in the biosynthesis of 3-deoxy-d-manno-octulosonate (KDO), a sugar moiety located in the lipopolysaccharide layer of most Gram-negative bacteria. Th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 2014
ISSN: 0014-5793
DOI: 10.1016/j.febslet.2014.02.023